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Image Search Results
Journal: Cell Reports Methods
Article Title: In situ microwave fixation provides an instantaneous snapshot of the brain metabolome
doi: 10.1016/j.crmeth.2023.100455
Figure Lengend Snippet:
Article Snippet:
Techniques:
Journal: Alcohol and alcoholism (Oxford, Oxfordshire)
Article Title: Glycosylation is altered by ethanol in rat hippocampal cultured neurons.
doi: 10.1093/alcalc/agl044
Figure Lengend Snippet: Fig. 4. Western blot analysis of the glucose transporters GLUT1 and GLUT3 in control (CNT) and alcohol-exposed (EtOH) neurons. Histograms show increases in densitometric readings of GLUT1 and GLUT3 in alcohol-exposed cells compared with the controls. The data used for the statistical analysis are the mean ± SD of four independent experiments. Asterisks indicate significant differences (Student’s t-test P < 0.05).
Article Snippet: Antibodies: anti-MAP2 mouse monoclonal antibody (Sigma-Aldrich, Spain); anti-neurofilament (NF) 200 rabbit polyclonal antibody (Sigma-Aldrich); anti-GLUT1 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc. CA, USA),
Techniques: Western Blot, Control
Journal: The Journal of Neuroscience
Article Title: Energy Requirements of Odor Transduction in the Chemosensory Cilia of Olfactory Sensory Neurons Rely on Oxidative Phosphorylation and Glycolytic Processing of Extracellular Glucose
doi: 10.1523/JNEUROSCI.2640-16.2017
Figure Lengend Snippet: Olfactory neuron cilia and sustentacular cell microvilli express the high affinity GLUT3 glucose transporter. A, Immunocytochemistry for GLUT3 without (left) or with (right) preincubation of the primary antibody (Alpha Diagnostics anti-GLUT3 antibody) with its antigenic peptide in rat OSNs, as a control for the specificity of the antibody. The left three panels show a neuron labeled for OMP, GLUT3, and a merge of both; the two right panels show the control. B, Immunocytochemistry (Sigma anti-GLUT3 antibody) for GLUT3 of an isolated rat OSN. C, Isolated toad OSN (Sigma anti-GLUT3 antibody). D, Rat SCs (Sigma anti-GLUT3 antibody). GLUT3-expressing cilia are indicated by arrowheads. C, D, Insets (3×) correspond to the areas denoted in the figures. Nuclei are labeled blue (TOPRO). Scale bars, 10 μm.
Article Snippet: D ,
Techniques: Immunocytochemistry, Labeling, Isolation, Expressing